Step 1: Select the appropriate service and sample type.

{ binding firstError.message }

{ binding firstError.message }

Learn about our RNA Sequencing services.

{ binding firstError.message }

Learn about our Whole Genome Sequencing services.

{ binding firstError.message }

Learn about our Amplicon Sequencing services.

{ binding firstError.message }

Learn about our Targeted Sequencing services.

{ binding firstError.message }

Learn about our Metagenomics services.

{ binding firstError.message }

Learn about our Epigenomics services.

{ binding firstError.message }

Learn more our Immunogenomics services.

Learn more about our Sequencing Only service.

Learn more about our Exome Sequencing service.

Learn more about our CRISPR Validation service.

{ binding firstError.message }
{ binding firstError.message }
{ binding firstError.message }
{ binding firstError.message }
{ binding firstError.message }
{ binding firstError.message }
{ binding firstError.message }
{ binding firstError.message }
{ binding firstError.message }
{ binding firstError.message }
{ binding firstError.message }

 

 

 

Step 2: Follow these guidelines when preparing and submitting your samples.

Cell Pellet

Sample Type: Cell Pellet

Recommended Amount: 

≥10^6 cells (eukaryotic); ≥10^8 cells (microorganism)

Minimum Amount:

10^4 cells (eukaryotic);10^6 cells (microorganism)

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Cell Pellet (genoTYPER-NEXT)

Sample Type: Cell Pellet

Recommended Amount: >1M cells

Minimum Amount: 30,000 cells

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Cells in Solution

Sample Type: Cells in Solution

Recommended Amount: >1,000 cells

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

 

 

Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq

Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.

Download >

 

{ binding firstError.message }

ChIP DNA

Sample Type: ChIP DNA (RNA-free)

Recommended Amount: >50 ng

Minimum Amount: 10 ng

Fragment Length: 100-800 bp, main peak at 200-300 bp

Purity (A260/280): 1.8-2.0

Buffer: Water, BE, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

FFPE

Sample Type: FFPE

Recommended Amount: ≥4 FFPE slides

Minimum Amount: 2 FFPE slides

Thickness: 5-20 µm

Area: >150 mm^2

Shipment Method: Room temperature or blue ice

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Fixed Needle Core Biopsy

Sample Type: Fixed Needle Core Biopsy

Recommended Amount: ≥4 biopsies

Minimum Amount: 1 biopsy

Diameter: 1-2 mm

Length: >5 mm

Shipment Method: Room temperature or blue ice

 

 

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

{ binding firstError.message }

Fresh Frozen Tissue

Sample Type: Fresh Frozen Tissue

Recommended Amount: >10 mg

Minimum Amount: 2 mg

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Single-Cell

Sample Type: Frozen cell suspension according to GENEWIZ-supplied protocol, submitted in duplicate. Methanol-fixed cells are also accepted.

Recommended Amount: >1M cells in 1 mL

Minimum Amount: 50,000 cells in 500 µL

Buffer: Cryopreservation media (e.g. cell culture media supplemented with 20% FBS and 10% DMSO)

 

Note: GENEWIZ has developed a proprietary cryopreservation protocol to aid shipment of samples.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

 

 

Tech Note: Add More Life to Your Data: Optimizing Single-Cell RNA-Seq with Dead Cell Removal

Obtaining samples with high cell viability can be difficult for many experiments but is necessary for success on the 10x Genomics® Chromium™ platform. This tech note describes how GENEWIZ scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data.

Download >

 

{ binding firstError.message }

ATAC-Seq Cells

Sample Type: Frozen cell suspension according to GENEWIZ-supplied protocol

Recommended Amount: >100,000 cells in 500 μL

Minimum Amount: 50,000 cells in 500 µL

Buffer: Cryopreservation media (e.g. cell culture media supplemented with 10% DMSO)

 

Note: GENEWIZ has developed a proprietary cryopreservation protocol to aid shipment of samples.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

{ binding firstError.message }

ATAC-Seq Cells

Sample Type: Frozen intact plant tissue is preferable. Tissue ground to a fine powder can be submitted after consultation with GENEWIZ.

Amount: Very little frozen tissue is required; the equivalent of a microcentrifuge tube filled with tissue should be sufficient

 

Note: Samples cannot be submitted in solution, such as RNAlater.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

{ binding firstError.message }

ATAC-Seq Cells

Sample Type: Frozen intact plant tissue is preferable. Tissue ground to a fine powder can be submitted after consultation with GENEWIZ.

Amount: 1 gram of frozen tissue is required for up to 4 immunoprecipitations (i.e. antibodies)

Note: Samples cannot be submitted in solution, such as RNAlater.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

{ binding firstError.message }

Genomic DNA

Sample Type: Genomic DNA (RNA-free)

Recommended Amount: >1.5 µg @ >20 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Genomic DNA PacBio

Sample Type: Genomic DNA (RNA-free)

Recommended Amount: >25 µg @ 100 ng/µL

Minimum Amount: 15 µg (singleplex), 5 µg (multiplex)

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

View our Best Practices Guide to Preparation of High Molecular Weight DNA​ 

{ binding firstError.message }

Genomic DNA (targeted amplicon)

Sample Type: Genomic DNA (RNA-free)

Recommended Amount: >1 µg @ >20 ng/µL

Minimum Amount: 100 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Plasma/Serum

Sample Type: Plasma/Serum

Recommended Amount: 2 tubes/sample, 10 mL/tube

Minimum Amount: 5 mL/tube

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Premade Sequencing Library

Sample Type: Premade Sequencing Library

Recommended Amount: >15 nM, >20 µL

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Shipment Method: Dry ice

{ binding firstError.message }

Purified Mixed DNA

Sample Type: Purified Mixed DNA (RNA-free)

Minimum Amount: 500 ng

Concentration: Normalized to 20 ng/µL

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Purified Mixed Genomic DNA

Sample Type: Purified Mixed Genomic DNA (RNA-free)

Recommended Amount: >1.5 µg @ >20 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Purified PCR Products

Sample Type: Purified PCR Products

Recommended Amount: >1.5 µg @ >20 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Purified PCR Products PacBio

Sample Type: Purified PCR Products for PacBio

Recommended Amount: >2 µg @ >50 ng/µL

Purity (A260/280): 1.8-2.0

Buffer: 10 mM Tris-HCl, pH 8

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

View our Best Practices Guide to Preparation of Amplicon DNA for PacBio Sequencing

{ binding firstError.message }

Amplicon-EZ

Sample Type: Purified PCR Products

Size: 150-500 bp

For optimal results, we recommend samples that produce only one band on a gel.

Minimum Amount: 500 ng

We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Concentration: Normalized to 20 ng/µL

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Submission Methods: 

  • Drop off samples in a GENEWIZ drop box at room temperature
  • Ship at room temperature or on blue ice

View our Amplicon-EZ Best Practices Checklist for more info

{ binding firstError.message }

Purified Small RNA

Sample Type: Purified Small RNA (DNA-free)

Recommended Amount: >50 ng @ >5 ng/µL

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥6.0

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Saliva

Sample Type: Saliva

Recommended Amount: 1 mL

Shipment Method: Dry ice or blue ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

{ binding firstError.message }

Snap Frozen Needle Core Biopsy

Sample Type: Snap Frozen Needle Core Biopsy

Recommended Amount: 10 mg

Minimum Amount: 3 mg

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

{ binding firstError.message }

Soil

Sample Type: Soil

Recommended Amount: 100 mg

Minimum Amount: 50 mg

Shipment Method: Room temperature or blue ice

{ binding firstError.message }

Stool

Sample Type: Stool (solid; no buffer)*

Recommended Amount: 100 mg

Minimum Amount: 50 mg

Shipment Method: Dry ice or blue ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

*If you would like to submit samples in buffer/solution, Azenta may still be able to proceed. Please contact us with the buffer composition/volume you have for how to proceed.

{ binding firstError.message }

Swab

Sample Type: Swab

Recommended Amount: 100 mg

Shipment Method: Room temperature

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

{ binding firstError.message }

Total RNA

Sample Type: Total RNA (DNA-free)

Recommended Amount: >2 µg @ >50 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥6.0

 

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

 

 

Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq

Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.

Download >

 

{ binding firstError.message }

Total RNA (Iso-Seq)

Sample Type: Total RNA (DNA-free)

Recommended Amount: >2 µg, 10-20 µL

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥9.0

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Total RNA (ultra-low input)

Sample Type: Total RNA (DNA-free)

Recommended Amount: 20 ng

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

 

 

Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq

Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.

Download >

 

{ binding firstError.message }

Total RNA (small RNA)

Sample Type: Total RNA, including small RNA (DNA-free)

Recommended Amount: >2 µg @ >50 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥6.0

 

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Water

Sample Type: Water (environmental)

Recommended Volume: 100 mL

Minimum Volume: 20 mL

Shipment Method: Room temperature

{ binding firstError.message }

Whole Blood (DNA)

Sample Type: Whole Blood (DNA extraction)

Recommended Amount: 2 tubes/sample, 500 µL/tube

Minimum Amount: 200 µL/tube

Shipment Method:

  • Blue ice for fresh blood
  • Dry ice for frozen blood

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Whole Blood (RNA)

Sample Type: Whole Blood (RNA extraction)

Recommended Volume: 2.5 mL in PAXgene Blood RNA Tube

Shipment Method:

  • Blue ice for fresh blood
  • Dry ice for frozen blood

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

< Start Over

Step 1: Select the appropriate service and sample type.

NGS Category {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }

RNA Sequencing Service {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }

Learn about our RNA Sequencing services.

Whole Genome Sequencing Service {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }

Learn about our Whole Genome Sequencing services.

Amplicon Sequencing Service {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }

Learn about our Amplicon Sequencing services.

Targeted Sequencing Service {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }

Learn about our Targeted Sequencing services.

Metagenomics Service {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }

Learn about our Metagenomics services.

Epigenomics Service {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }

Learn about our Epigenomics services.

Immunogenomics Services {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }

Learn more our Immunogenomics services.

Learn more about our Sequencing Only service.

Learn more about our Exome Sequencing service.

Learn more about our CRISPR Validation service.

Sample Type (RNA-Seq) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }
Sample Type (Small RNA-Seq) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }
Sample Type (Ultra-Low Input RNA-Seq) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }
Sample Type (Whole Genome) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }
Sample Type (Targeted/Exome) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }
Sample Type (Whole Genome Metagenomics) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }
Sample Type (genoTYPER-NEXT) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }
Sample Type (16S-EZ/Fungal ID) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }
Sample Type (Bulk Immuno-Profiling) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }
Sample Type (ATAC-Seq) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }
Sample Type (ChIP-Seq) {{ Cognito.resources["required-asterisk"] }} , { binding firstError.message }
{binding displayValue}
{ binding firstError.message }

 

 

 

Step 2: Follow these guidelines when preparing and submitting your samples.

Cell Pellet

Sample Type: Cell Pellet

Recommended Amount: 

≥10^6 cells (eukaryotic); ≥10^8 cells (microorganism)

Minimum Amount:

10^4 cells (eukaryotic);10^6 cells (microorganism)

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Cell Pellet (genoTYPER-NEXT)

Sample Type: Cell Pellet

Recommended Amount: >1M cells

Minimum Amount: 30,000 cells

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Cells in Solution

Sample Type: Cells in Solution

Recommended Amount: >1,000 cells

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

 

 

Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq

Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.

Download >

 

{ binding firstError.message }

ChIP DNA

Sample Type: ChIP DNA (RNA-free)

Recommended Amount: >50 ng

Minimum Amount: 10 ng

Fragment Length: 100-800 bp, main peak at 200-300 bp

Purity (A260/280): 1.8-2.0

Buffer: Water, BE, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

FFPE

Sample Type: FFPE

Recommended Amount: ≥4 FFPE slides

Minimum Amount: 2 FFPE slides

Thickness: 5-20 µm

Area: >150 mm^2

Shipment Method: Room temperature or blue ice

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Fixed Needle Core Biopsy

Sample Type: Fixed Needle Core Biopsy

Recommended Amount: ≥4 biopsies

Minimum Amount: 1 biopsy

Diameter: 1-2 mm

Length: >5 mm

Shipment Method: Room temperature or blue ice

 

 

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

{ binding firstError.message }

Fresh Frozen Tissue

Sample Type: Fresh Frozen Tissue

Recommended Amount: >10 mg

Minimum Amount: 2 mg

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Single-Cell

Sample Type: Frozen cell suspension according to GENEWIZ-supplied protocol, submitted in duplicate. Methanol-fixed cells are also accepted.

Recommended Amount: >1M cells in 1 mL

Minimum Amount: 50,000 cells in 500 µL

Buffer: Cryopreservation media (e.g. cell culture media supplemented with 20% FBS and 10% DMSO)

 

Note: GENEWIZ has developed a proprietary cryopreservation protocol to aid shipment of samples.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

 

 

Tech Note: Add More Life to Your Data: Optimizing Single-Cell RNA-Seq with Dead Cell Removal

Obtaining samples with high cell viability can be difficult for many experiments but is necessary for success on the 10x Genomics® Chromium™ platform. This tech note describes how GENEWIZ scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data.

Download >

 

{ binding firstError.message }

ATAC-Seq Cells

Sample Type: Frozen cell suspension according to GENEWIZ-supplied protocol

Recommended Amount: >100,000 cells in 500 μL

Minimum Amount: 50,000 cells in 500 µL

Buffer: Cryopreservation media (e.g. cell culture media supplemented with 10% DMSO)

 

Note: GENEWIZ has developed a proprietary cryopreservation protocol to aid shipment of samples.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

{ binding firstError.message }

ATAC-Seq Cells

Sample Type: Frozen intact plant tissue is preferable. Tissue ground to a fine powder can be submitted after consultation with GENEWIZ.

Amount: Very little frozen tissue is required; the equivalent of a microcentrifuge tube filled with tissue should be sufficient

 

Note: Samples cannot be submitted in solution, such as RNAlater.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

{ binding firstError.message }

ATAC-Seq Cells

Sample Type: Frozen intact plant tissue is preferable. Tissue ground to a fine powder can be submitted after consultation with GENEWIZ.

Amount: 1 gram of frozen tissue is required for up to 4 immunoprecipitations (i.e. antibodies)

Note: Samples cannot be submitted in solution, such as RNAlater.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice overnight

{ binding firstError.message }

Genomic DNA

Sample Type: Genomic DNA (RNA-free)

Recommended Amount: >1.5 µg @ >20 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Genomic DNA PacBio

Sample Type: Genomic DNA (RNA-free)

Recommended Amount: >25 µg @ 100 ng/µL

Minimum Amount: 15 µg (singleplex), 5 µg (multiplex)

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

View our Best Practices Guide to Preparation of High Molecular Weight DNA​ 

{ binding firstError.message }

Genomic DNA (targeted amplicon)

Sample Type: Genomic DNA (RNA-free)

Recommended Amount: >1 µg @ >20 ng/µL

Minimum Amount: 100 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

Need additional samples for your cohort study? Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Plasma/Serum

Sample Type: Plasma/Serum

Recommended Amount: 2 tubes/sample, 10 mL/tube

Minimum Amount: 5 mL/tube

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Premade Sequencing Library

Sample Type: Premade Sequencing Library

Recommended Amount: >15 nM, >20 µL

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Shipment Method: Dry ice

{ binding firstError.message }

Purified Mixed DNA

Sample Type: Purified Mixed DNA (RNA-free)

Minimum Amount: 500 ng

Concentration: Normalized to 20 ng/µL

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Purified Mixed Genomic DNA

Sample Type: Purified Mixed Genomic DNA (RNA-free)

Recommended Amount: >1.5 µg @ >20 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Purified PCR Products

Sample Type: Purified PCR Products

Recommended Amount: >1.5 µg @ >20 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Purified PCR Products PacBio

Sample Type: Purified PCR Products for PacBio

Recommended Amount: >2 µg @ >50 ng/µL

Purity (A260/280): 1.8-2.0

Buffer: 10 mM Tris-HCl, pH 8

Note: We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

View our Best Practices Guide to Preparation of Amplicon DNA for PacBio Sequencing

{ binding firstError.message }

Amplicon-EZ

Sample Type: Purified PCR Products

Size: 150-500 bp

For optimal results, we recommend samples that produce only one band on a gel.

Minimum Amount: 500 ng

We recommend using a dsDNA quantification assay such as Qubit or PicoGreen.

Concentration: Normalized to 20 ng/µL

Purity (A260/280): 1.8-2.0

Buffer: Water, EB, or low TE (<0.1 mM EDTA)

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Submission Methods: 

  • Drop off samples in a GENEWIZ drop box at room temperature
  • Ship at room temperature or on blue ice

View our Amplicon-EZ Best Practices Checklist for more info

{ binding firstError.message }

Purified Small RNA

Sample Type: Purified Small RNA (DNA-free)

Recommended Amount: >50 ng @ >5 ng/µL

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥6.0

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Saliva

Sample Type: Saliva

Recommended Amount: 1 mL

Shipment Method: Dry ice or blue ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

{ binding firstError.message }

Snap Frozen Needle Core Biopsy

Sample Type: Snap Frozen Needle Core Biopsy

Recommended Amount: 10 mg

Minimum Amount: 3 mg

Shipment Method: Dry ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

{ binding firstError.message }

Soil

Sample Type: Soil

Recommended Amount: 100 mg

Minimum Amount: 50 mg

Shipment Method: Room temperature or blue ice

{ binding firstError.message }

Stool

Sample Type: Stool (solid; no buffer)*

Recommended Amount: 100 mg

Minimum Amount: 50 mg

Shipment Method: Dry ice or blue ice

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

*If you would like to submit samples in buffer/solution, Azenta may still be able to proceed. Please contact us with the buffer composition/volume you have for how to proceed.

{ binding firstError.message }

Swab

Sample Type: Swab

Recommended Amount: 100 mg

Shipment Method: Room temperature

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

{ binding firstError.message }

Total RNA

Sample Type: Total RNA (DNA-free)

Recommended Amount: >2 µg @ >50 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥6.0

 

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

 

 

Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq

Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.

Download >

 

{ binding firstError.message }

Total RNA (Iso-Seq)

Sample Type: Total RNA (DNA-free)

Recommended Amount: >2 µg, 10-20 µL

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥9.0

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Total RNA (ultra-low input)

Sample Type: Total RNA (DNA-free)

Recommended Amount: 20 ng

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

 

 

Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq

Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.

Download >

 

{ binding firstError.message }

Total RNA (small RNA)

Sample Type: Total RNA, including small RNA (DNA-free)

Recommended Amount: >2 µg @ >50 ng/µL

Minimum Amount: 500 ng

Purity (A260/280): 1.8-2.2

Recommended RIN: ≥6.0

 

Buffer: Nuclease-free water

Tubes/Plates: For projects with <36 samples, prepare samples in clearly labeled microcentrifuge tubes. For larger projects, use 96-well PCR plates.

Shipment Method: Dry ice

{ binding firstError.message }

Water

Sample Type: Water (environmental)

Recommended Volume: 100 mL

Minimum Volume: 20 mL

Shipment Method: Room temperature

{ binding firstError.message }

Whole Blood (DNA)

Sample Type: Whole Blood (DNA extraction)

Recommended Amount: 2 tubes/sample, 500 µL/tube

Minimum Amount: 200 µL/tube

Shipment Method:

  • Blue ice for fresh blood
  • Dry ice for frozen blood

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

Whole Blood (RNA)

Sample Type: Whole Blood (RNA extraction)

Recommended Volume: 2.5 mL in PAXgene Blood RNA Tube

Shipment Method:

  • Blue ice for fresh blood
  • Dry ice for frozen blood

Note: GENEWIZ can work with BSL1 and some BSL2 specimens.

Need additional samples for your cohort study? 

Get streamlined access to Nashville Bio's world-class biobank

{ binding firstError.message }

< Start Over